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KMID : 0043319920150010058
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Archives of Pharmacal Research
1992 Volume.15 No. 1 p.58 ~ p.61
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A Macrolide-Lincosaminde-Streptogramin B Resistance Determinant Gene(ermJ) Cloned from B. anthracis 590
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Kim HS
Choi EC/Kim BK/Park YI
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Abstract
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Bacillus anthracis 590 having an inducible resistance determinant to MLS antibiotics was isolated from a soil sample in Korea. The resistance gene (ermJ) was cloned by Southern blotting of chromosomal DNA fragment digested by various restriction enzymes and coloy hybridization method and the cloned plasmid was named as pBA423. The size of inserted DNA fragment to pBS42 vector was about 2.9kb and the DNA sequence of the subcloned fragment (Hinc II-Hinc II, 1.4kb) was determined. The DNA sequence of ermJ was composed of 357 bp for leader region and 861 bp for the structural gene. Because the leader sequence of ermJ was homologous to that of ermJ, the expression of ermJ is also thought to be controlled by a transcriptionl attenuation mechanism.
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